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primescripttm one step rt pcr kit ver  (TaKaRa)


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    Structured Review

    TaKaRa primescripttm one step rt pcr kit ver
    Primescripttm One Step Rt Pcr Kit Ver, supplied by TaKaRa, used in various techniques. Bioz Stars score: 96/100, based on 666 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primescripttm+one+step+rt+pcr+kit+ver/PrimeScript+One+Step+RT-PCR+Kit+Ver%2E2/pmc12846610-84-14-20
    Average 96 stars, based on 666 article reviews
    primescripttm one step rt pcr kit ver - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Reverse Transcription:

    Article Title: Gonadal Transcriptome Analysis Reveals the lncRNA–mRNA Pair in Sea Cucumber Holothuria leucospilota
    Article Snippet: .. Total RNA (1 μg) underwent reverse transcription with the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, Tokyo, Japan) following the manufacturer’s protocol, involving a 60 min incubation at 37 °C followed by 5 s enzyme inactivation at 85 °C. .. Quantitative real-time PCR amplification was performed using Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, Carlsbad, CA, USA) with lncRNA-specific primers , using β-actin as the endogenous control [ ].

    One Step RT-PCR:

    Article Title: Gonadal Transcriptome Analysis Reveals the lncRNA–mRNA Pair in Sea Cucumber Holothuria leucospilota
    Article Snippet: .. Total RNA (1 μg) underwent reverse transcription with the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, Tokyo, Japan) following the manufacturer’s protocol, involving a 60 min incubation at 37 °C followed by 5 s enzyme inactivation at 85 °C. .. Quantitative real-time PCR amplification was performed using Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, Carlsbad, CA, USA) with lncRNA-specific primers , using β-actin as the endogenous control [ ].

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [ ]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: RNA virome of ticks and fleas infesting Marmota himalayana from the Qinghai-Tibet plateau.
    Article Snippet: .. To explore the potential presence of the M fragment, polymerase chain reaction was conducted using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, AR TIC LE IN PR ES S Shimogyō-ku, Kyot). ..

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLVspecific primers containing the T7 promoter sequence and poly(T) tail (Table S1). ..

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [16]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Preparation of a camel-canine chimeric antibody against canine B-cell lymphoma
    Article Snippet: Finally, the samples were examined under a confocal microscope (ZEISS, LSM − 800) to visualize the fluorescence signals. .. The Fc fragment of the canine IgG-B antibody was amplified using the total canine RNA extracted from canine PBMCs as a template and using Fc 1 and Fc (Table ) as primers with the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara Bio, Japan). .. The canine IgG-B Fc (cFc) fragment was ligated into the pFUSE-hIgG4-fc2 vector (InvivoGen, San Diego, USA) by restriction with Bgl II and Nhe I (Takara Bio, Japan) to replace the human hIgG4-Fc fragment.

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLV-specific primers containing the T7 promoter sequence and poly(T) tail ( ). ..

    Article Title: RNA virome of ticks and fleas infesting Marmota himalayana from the Qinghai-Tibet plateau
    Article Snippet: .. To explore the potential presence of the M fragment, polymerase chain reaction was conducted using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, Shimogyō-ku, Kyot). ..

    Incubation:

    Article Title: Gonadal Transcriptome Analysis Reveals the lncRNA–mRNA Pair in Sea Cucumber Holothuria leucospilota
    Article Snippet: .. Total RNA (1 μg) underwent reverse transcription with the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, Tokyo, Japan) following the manufacturer’s protocol, involving a 60 min incubation at 37 °C followed by 5 s enzyme inactivation at 85 °C. .. Quantitative real-time PCR amplification was performed using Platinum SYBR Green qPCR SuperMix-UDG (Invitrogen, Carlsbad, CA, USA) with lncRNA-specific primers , using β-actin as the endogenous control [ ].

    Extraction:

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [ ]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [16]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Amplification:

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [ ]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [16]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Preparation of a camel-canine chimeric antibody against canine B-cell lymphoma
    Article Snippet: Finally, the samples were examined under a confocal microscope (ZEISS, LSM − 800) to visualize the fluorescence signals. .. The Fc fragment of the canine IgG-B antibody was amplified using the total canine RNA extracted from canine PBMCs as a template and using Fc 1 and Fc (Table ) as primers with the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara Bio, Japan). .. The canine IgG-B Fc (cFc) fragment was ligated into the pFUSE-hIgG4-fc2 vector (InvivoGen, San Diego, USA) by restriction with Bgl II and Nhe I (Takara Bio, Japan) to replace the human hIgG4-Fc fragment.

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [ ]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLVspecific primers containing the T7 promoter sequence and poly(T) tail (Table S1). ..

    Article Title: Phylogenetic Analyses and Biological Characterization of H9N2 Avian Influenza Virus Isolated from Chickens in China from 2022 to 2023
    Article Snippet: .. The viral RNA was extracted from allantoic fluid using TaKaRa MiniBEST Viral RNA/DNA Extraction Kit Ver.5.0 (Takara Biomedical Technology, Beijing, China), and the genes coding sequences were amplified by RT-PCR using PrimeScriptTM One Step RT-PCR Kit Ver.2 (Dye Plus) (TaKaRa, Beijing, China) with specific primers [16]. .. The amplified products were sent to the BGI Tech Solutions (Beijing liuhe) Co., Ltd., Beijing, China for sequencing.

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLV-specific primers containing the T7 promoter sequence and poly(T) tail ( ). ..

    Polymerase Chain Reaction:

    Article Title: RNA virome of ticks and fleas infesting Marmota himalayana from the Qinghai-Tibet plateau.
    Article Snippet: .. To explore the potential presence of the M fragment, polymerase chain reaction was conducted using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, AR TIC LE IN PR ES S Shimogyō-ku, Kyot). ..

    Article Title: RNA virome of ticks and fleas infesting Marmota himalayana from the Qinghai-Tibet plateau
    Article Snippet: .. To explore the potential presence of the M fragment, polymerase chain reaction was conducted using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, Shimogyō-ku, Kyot). ..

    In Vitro:

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLVspecific primers containing the T7 promoter sequence and poly(T) tail (Table S1). ..

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLV-specific primers containing the T7 promoter sequence and poly(T) tail ( ). ..

    Sequencing:

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLVspecific primers containing the T7 promoter sequence and poly(T) tail (Table S1). ..

    Article Title: Transcriptomic and Epitranscriptomic Landscape of Integrated HTLV-1 in MT2 Cells
    Article Snippet: .. The DNA templates for in vitro transcription (IVT) were prepared by RT-PCR using the PrimeScriptTM One Step RT-PCR Kit Ver.2 (Takara, RR055A), with six pairs of HTLV-specific primers containing the T7 promoter sequence and poly(T) tail ( ). ..



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